|
KMID : 0615720100270030065
|
|
Jouranl of Korean Acupuncture and Moxibustion society
2010 Volume.27 No. 3 p.65 ~ p.73
|
|
|
Effects of Ampelopsis Radix Pharmacopuncture Solution on Nitric Oxide and Prostaglandin E2 Production in Macrophages
|
|
Kim Min-Seok
Roh Jeong-Du
|
|
|
Abstract
|
|
|
Objectives : Recently, Pharmacopuncture therapy has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, nitric oxide. The purpose of this study is investigated that the effect of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages, performed several experimental items : those are Prostaglandin E2, Nitric Oxide and Cyclooxygenase-2.
Methods : The cytotoxicity of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages were measured by MTT assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin E2 production and Nitric Oxide production was measured by nitric oxide detection kit and Prostaglandin E2 assay kit.
Results : 1. The MTT assay demonstrated that cytotoxic effect of Ampelopsis Radix Pharmacopuncture solution in RAW 264.7 macrophages was not appeared. 2. Ampelopsis Radix Pharmacopuncture solution inhibited nitric oxide production in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages. 3. Ampelopsis Radix Pharmacopuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide and interferon-gamma stimulated RAW 264.7 macrophages. 4. Ampelopsis Radix Pharmacopuncture solution inhibited Prostaglandin E2 production in lipopolysac- charide and interferon-gamma stimulated RAW 264.7 macrophages.
Conclusions : On the basis of these results, It was shown that Ampelopsis Radix Pharmacopuncture solution was able to inhibit the production of PGE2 and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Ampelopsis Radix Pharmacopuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.
|
|
|
KEYWORD
|
|
|
Ampelopsis Radix, Pharmacopuncture, Anti-inflammatory, NO, COX-2, PGE2, Korean Medici
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
|
|